The Golden Age of Directed Evolution of Enzymes

THE GOLDEN AGE OF DIRECTED EVOLUTION OF ENZYMES. Directed enzyme evolution is leaving a golden age. The consolidation of robust mutant library creation methods and the arise of reliable high-throughput screening assays have allowed scientists all over the world to generate synthetic proteins with functionalities that surpass by far those of natural counterparts. Importantly, he nascent association between Rosetta design and phylogenetic inference methods is assisting directed evolution enterprises. Along these lines, the use of paleoenzymology -ancestral sequence reconstruction and resurrection- aims at opening unexplored avenues for the laboratory evolution of more promiscuous and stable ancestral enzymes. In this lecture, I will discuss all these advances in this fascinating ground of research commenting several case studies from our work on peroxygenase and laccase engineering for the pharma, chemical and environmental sectors.

Miguel Alcalde

Full CSIC Professor

Instituto de Catálisis y Petroleoquímica, ICP, CSIC

Miguel Alcalde is CSIC Research Professor and founder of EvoEnzyme S.L. (https://evoenzyme.com), a spin-off stemming from his research group.

The central research of Dr. Miguel Alcalde primarily focusses on the engineering of enzymes by directed evolution for a wide range of biotechnological purposes as well as synthetic biology studies for environmental, energy and industrial applications (https://miguelalcaldelab.eu).

Dr. Alcalde is a biologist by training, with postdoctoral stay at CALTECH in the group of Frances Arnold (Nobel laurate in Chemistry 2018). Coauthor of over 100 articles on enzyme engineering and applied biocatalysis and 14 filled patents, Prof. Alcalde has supervised 35 research projects and 12 PhD Thesis. He was working as a manager of National Research Agency of Spain in the area of biotechnology, (2019-2021) and was in charge of coordinating the section of molecular biotechnology and synthetic biology of SEBBM (Spanish Society of Biochemistry and Molecular Biology, 2017-2020). He is current board member of the Biocatalysis division of the European Federation of Biotechnology (EFB).

Key:

Complete
Failed
Available
Locked
The Golden Age of Directed Evolution of Enzymes
Open to view video.
Open to view video. Directed enzyme evolution is leaving a golden age. The consolidation of robust mutant library creation methods and the arise of reliable high-throughput screening assays have allowed scientists all over the world to generate synthetic proteins with functionalities that surpass by far those of natural counterparts. Importantly, he nascent association between Rosetta design and phylogenetic inference methods is assisting directed evolution enterprises. Along these lines, the use of paleoenzymology -ancestral sequence reconstruction and resurrection- aims at opening unexplored avenues for the laboratory evolution of more promiscuous and stable ancestral enzymes. In this lecture, I will discuss all these advances in this fascinating ground of research commenting several case studies from our work on peroxygenase and laccase engineering for the pharma, chemical and environmental sectors.
You must register to access.